Sunan samfur: Baohuoside I foda 98%
Tushen Botanical: Epimedium koreanum Nakai, Epimedium brevicornu Maxim
CASNo:113558-15-9
Wani Suna:Icarside-II,Icarin-II
Takaddun bayanai: ≥98%
Launi:Rawaya mai haskefoda tare da halayyar wari da dandano
GMOMatsayi: GMO Kyauta
Shiryawa: a cikin 25kgs fiber ganguna
Adana: Ajiye akwati a buɗe a wuri mai sanyi, busasshiyar wuri, Nisantar haske mai ƙarfi
Rayuwar Shelf: watanni 24 daga ranar samarwa
Baohuoside I wani fili ne na flavonoid da aka samu daga Epimedium koreanum.A matsayin mai hanawa na CXCR4, zai iya hana maganganun CXCR4, haifar da apoptosis, kuma yana da aikin rigakafin ciwon daji.
Ana samun foda na Baohuoside daga Epimedium koreanum Nakai ko Epimedium brevicornu Maxim, wani tsiro na ganye a China, Asiya.Tsarin kera na Baohuoside yana farawa da ɗanyen kayan da ake samu daga shukar Epimedium ana niƙasa sannan a fitar da shi da ethanol.Ruwan da aka fitar ana tacewa kuma a tattara shi kafin a diluting da ruwa kuma a yi amfani da hydrolysis na enzymatic.Bayan haka, ana wanke abun kuma a zubar da shi cikin ethanol, sannan a bi da hankali, cirewar ƙarfi, dawo da sauran ƙarfi, crystallization, tacewa, da bushewa wanda a ƙarshe ya samar da foda na Baohuoside 98% a cikin foda na ƙarshe.Dole ne a kula da hankali ga kowane mataki yayin sarrafa Baohuoside saboda aikinsu na musamman yana taimakawa ƙirƙirar samfur wanda zai iya riƙe fa'idodin lafiyarsa yadda yakamata a duk tsawon rayuwar sa idan an adana shi da kyau.A ƙarshe masana'antun Baohuoside suna samar da ƙarin ƙarin mahimmanci tare da kewayon tasiri mai kyau akan lafiyar mutum idan aka yi amfani da shi daidai.
In VitroAyyuka:Baohuoside I shine mai hana CXCR4 kuma yana rage ka'idojin CXCR4 a 12-25μ M. Baohuoside I (0-25μ M) yana hana NF -κ Kunna B ta hanyar dogaro da kashi kuma yana hana CXCL12 mamayewar ƙwayoyin cutar kansar mahaifa.Bohorside na kuma hana mamaye kwayoyin cutar kansar nono [1].Baohuoside I ya hana yiwuwar tantanin halitta A549, tare da ƙimar IC50 na 25.1μ M a 24 hours, 11.5μ M, da 9.6μ M a 48 hours da 72 hours, bi da bi.Bohorside I (25μ M) yana hana caspase cascade a cikin ƙwayoyin A549, yana ƙaruwa matakan ROS, kuma yana kunna JNK da p38MAPK siginar cascades [2].Boforseid I (3.125, 6.25, 12.5, 25.0, da 50.0μ g/mL) mahimmanci kuma kashi-dogara ya toshe haɓakar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar cuta ta Eca109, tare da IC50 na 4.8μ g/ml a 48 hours [3].
A cikin Ayyukan Vivo:Baohuoside I (25 mg/kg) na iya rage matakanβ - furotin catenin a cikin mice tsirara, Maganar cyclin D1 da survivin
Gwajin kwayoyin halitta:
Sakamakon cytotoxic na Baohuoside I akan ƙwayoyin A549 an ƙaddara ta hanyar MTT.Inoculate Kwayoyin (1 × 10 4 Kwayoyin / rijiya) a cikin wani farantin rijiyar 96 da kuma bi da Baohua glycoside I (6.25, 12.5, da kuma 25 μ M) ko 1mM NAC na 24, 48, ko 72 hours.Bayan cire matsakaicin al'adun da ke ɗauke da MTT, narkar da lu'ulu'u da aka kafa ta ƙara DMSO zuwa kowace rijiya.Bayan haɗawa, auna ɗaukar sel a 540 nm ta amfani da Multiskan Spectrum Microplate Reader [2].
Gwajin dabbobi:
An yi amfani da berayen Balb/c tsirara (makonni 4-6) don aunawa.Harvest Eca109 Luc Kwayoyin daga sub confluence kuma mayar da su a cikin PBS har zuwa karshe yawa ne 2 × 107 cell/mL.Kafin allura, sake dakatar da sel a cikin PBS kuma bincika su ta amfani da 0.4% trypan blue exclusion assay (rayyyu cell>90%).Don allurar subcutaneous, 1 × 107 Eca109 Luc Kwayoyin daga 200 μ LPBS an yi musu allura zuwa cikin hagu na kowane linzamin kwamfuta ta amfani da allura 27G.Bayan sati daya na allurar kwayar cutar tumo, an yi wa Boforside I (25mg/kg a kowace linzamin kwamfuta) allurar a cikin raunin sau daya a rana, yayin da mice 10 da aka yi amfani da su don maganin vector an ba su daidai adadin PBS [3].
